Somatic embryogenesis from cell cultures of Medicago sativa LI The role of amino acid additions to the regeneration medium

Tipo de material: Texto

TextoSeries ; Plant Science Letters, 34, p.165-174, 1984Trabajos contenidos:

Stuart, D.A
Strickland, S.G

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Resumen: The effect of adding amino acids to cultures of alfalfa (Medicago sativa L.)undergoing in vitro somatic embryogenesis has been investigated. By supplementing Schenk-Hildebrandt (SH)medium with 50--300 mM L-proline, somatic embryogenesis was stimulated by 3-fold when compared with SH medium without proline. Several other proline analogs were also effective. Alanine, glutamine, arginine, lysine, serine, asparagine and ornithine also stimulated the numbers of somatic embryos formed in alfalfa cultures. The structural quality of embryos was also enhanced by glutamine, arginine or alanine in the regeneration medium. Both embryo size and conversion of embryos to plantlets were increased by these amino acids. It is suggested that adding proline with other amino acids which stimulate embryo conversion may be one method for achieving high frequency and high quality somatic embryogenesis.

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The effect of adding amino acids to cultures of alfalfa (Medicago sativa L.)undergoing in vitro somatic embryogenesis has been investigated. By supplementing Schenk-Hildebrandt (SH)medium with 50--300 mM L-proline, somatic embryogenesis was stimulated by 3-fold when compared with SH medium without proline. Several other proline analogs were also effective. Alanine, glutamine, arginine, lysine, serine, asparagine and ornithine also stimulated the numbers of somatic embryos formed in alfalfa cultures. The structural quality of embryos was also enhanced by glutamine, arginine or alanine in the regeneration medium. Both embryo size and conversion of embryos to plantlets were increased by these amino acids. It is suggested that adding proline with other amino acids which stimulate embryo conversion may be one method for achieving high frequency and high quality somatic embryogenesis.

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