Evidence that Discontinuous DNA Replication in Escherichia coli is Primed by Approximately 10 to 12 Residues of RNA Starting with a Purine

Tipo de material: Texto

TextoSeries ; J. Mol. Biol., 184(1), p.45-52, 1985Trabajos contenidos:

Kitanit, T
Yoda, K
Ogawa, T
Okazaki, T

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Resumen: Intact primer RNA for discontinuous DNA replication of Eseheriehiu cohas been detected by specific labeling in vitro of its Fit-terminal tri- (or di-)phosphate group with vaccinia guanylyltransferase and [~z-~*P]GTP. A mutant defective either in RNase H or in bot'h RNase H and DNA polymerase I accumulated about 10 or 30 times more intact primer RNA, respectively, than wild-type cells. The primers started with purine in an A to G rat,io of 5 and the most abundant 5'-terminal dinucleotide sequence was (p)ppA-Pu. The chain length of the intact primer RNA was approximately 10 to 12 nucleotide residues. The structural properties of the E. coli primer RNA resemble those of the eukaryotic primer RNA.

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Intact primer RNA for discontinuous DNA replication of Eseheriehiu cohas been detected by specific labeling in vitro of its Fit-terminal tri- (or di-)phosphate group with vaccinia guanylyltransferase and [~z-~*P]GTP. A mutant defective either in RNase H or in bot'h RNase H and DNA polymerase I accumulated about 10 or 30 times more intact primer RNA, respectively, than wild-type cells. The primers started with purine in an A to G rat,io of 5 and the most abundant 5'-terminal dinucleotide sequence was (p)ppA-Pu. The chain length of the intact primer RNA was approximately 10 to 12 nucleotide residues. The structural properties of the E. coli primer RNA resemble those of the eukaryotic primer RNA.

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