A New Method of Sequencing DNA

Tipo de material: Texto

TextoSeries ; Analytical BioChemistry, 174(2), p.423-436, 1988Trabajos contenidos:

Hyman, E.D

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Resumen: An entirely new method of sequencing DNA has been devised that does not use electrophoresis, radioactivity, or fluorescence. The method works by measuring pyrophosphate generated by the DNA polymerization reaction. DNA and DNA polymerase are held by a DEAE-Sepharose column and solutions containing different dNTPs are pumped through. The pyrophosphate generated is measured continuously by a device consisting of a series of columns containing enzymes covalently attached to Sepharose. The alternating copolymer poly(dA.dT)is sequenced as an illustration of the method. Future improvements that will facilitate automation are discussed

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An entirely new method of sequencing DNA has been devised that does not use electrophoresis, radioactivity, or fluorescence. The method works by measuring pyrophosphate generated by the DNA polymerization reaction. DNA and DNA polymerase are held by a DEAE-Sepharose column and solutions containing different dNTPs are pumped through. The pyrophosphate generated is measured continuously by a device consisting of a series of columns containing enzymes covalently attached to Sepharose. The alternating copolymer poly(dA.dT)is sequenced as an illustration of the method. Future improvements that will facilitate automation are discussed

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