Callus formation and shoot regeneration in vanilla planifolia

Tipo de material: Texto

TextoSeries ; Food Biotechnology, 5(1), p.59-66, 1991Trabajos contenidos:

Davidonis, G
Knorr, D

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Resumen: The high demand for natural vanilla flavor led to attempts to establish an in vitro cell culture of Vanilla planifolia for flavor production and for shoot regeneration from callus. Explants from Vanilla planifolia shoots were placed on supplement Murashige and Skoog salts and tested for callus formation. Callus formation could be achieved with various media. Shoots could be regenerated from callus under various concentration sofa-naphthalene acetic acid(NAA)and benzyladenine(BA).The first no dewas the best location for callus initiation with subsequent callus proliferation.

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The high demand for natural vanilla flavor led to attempts to establish an in vitro cell culture of Vanilla planifolia for flavor production and for shoot regeneration from callus. Explants from Vanilla planifolia shoots were placed on supplement Murashige and Skoog salts and tested for callus formation. Callus formation could be achieved with various media. Shoots could be regenerated from callus under various concentration sofa-naphthalene acetic acid(NAA)and benzyladenine(BA).The first no dewas the best location for callus initiation with subsequent callus proliferation.

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