Transformation of Yeast by Lithium Acetate/Single-Stranded Carrier DNA/Polyethylene Glycol Method

Tipo de material: Texto

TextoSeries ; Methods in Enzymology, 350, p.87-96, 2002Trabajos contenidos:

Daniel Gietz, R
Woods, R.A

Recursos en línea:

Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx

Resumen: The introduction of exogenous DNA into yeast by transformation has become an essential technique in molecular biology. Transformation is used to investigate the genomics and proteomics of yeast itself and also when yeast is employed as a system to study the genes and gene products of other organisms. Intact yeast cells can be transformed by a number of procedures: the lithium acetate/single-stranded carrier DNA/polyethylene glycol (LiAc/SS-Carrier DNA/PEG)method, 1-3 elec- troporation, 4-6 agitation with glass beads, 7 and bombardment with DNA-coated microprojectiles. 8 Cells can also be transformed after conversion to spheroplasts by treatment with Zymolyase. 9 We have reviewed the application of these techniques to Saccharomyces cerevisiae and other yeastsJ ° We focus here on the transforma- tion of intact cells by the LiAc/SS-Carrier DNA/PEG method since it is the most widely applicable.

Tags from this library: No tags from this library for this title. Log in to add tags.

Average rating: 0.0 (0 votes)

Holdings
Item type	Current library	Collection	Call number	Status	Date due	Barcode
Documentos solicitados	CICY Documento préstamo interbibliotecario	Ref1	B-14853 (Browse shelf(Opens below))	Available

Browsing CICY shelves, Shelving location: Documento préstamo interbibliotecario, Collection: Ref1 Close shelf browser (Hides shelf browser)

Previous	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	Next
Previous	B-14850 The 'C' ring test for the strength of brittle materials	B-14851 Isolation form Argemone oil of Dihydrosanguinarine and sanguinarine: toxicity of sanguinarine	B-14852 Molecular Dynamics Simulations of Diffusion of O2and N2Penetrants in Polydimethylsiloxane-Based Nanocomposites	B-14853 Transformation of Yeast by Lithium Acetate/Single-Stranded Carrier DNA/Polyethylene Glycol Method	B-14854 Enrichment planting in a tropical secondary forest in Veracruz, Mexico	B-14855 Environmental management Life cycle assessment requirements and guidelines	B-14856 Environmental management life cycle assessment principles and framework. la sustituye: Gestión ambiental Análisis del ciclo de vida principios y marco de referencia	Next

The introduction of exogenous DNA into yeast by transformation has become an essential technique in molecular biology. Transformation is used to investigate the genomics and proteomics of yeast itself and also when yeast is employed as a system to study the genes and gene products of other organisms. Intact yeast cells can be transformed by a number of procedures: the lithium acetate/single-stranded carrier DNA/polyethylene glycol (LiAc/SS-Carrier DNA/PEG)method, 1-3 elec- troporation, 4-6 agitation with glass beads, 7 and bombardment with DNA-coated microprojectiles. 8 Cells can also be transformed after conversion to spheroplasts by treatment with Zymolyase. 9 We have reviewed the application of these techniques to Saccharomyces cerevisiae and other yeastsJ ° We focus here on the transforma- tion of intact cells by the LiAc/SS-Carrier DNA/PEG method since it is the most widely applicable.

There are no comments on this title.

to post a comment.