DNA Sequencing: An Outsourcing Guide

Tipo de material: Texto

TextoSeries ; Current Protocols Essential Laboratory Techniques, 2(1), p.12-1, 2009Trabajos contenidos:

Touchman, J.W

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Resumen: DNA sequencing, the process of determining the precise order of nucleotides in a DNA strand, is a fundamental and ubiquitous method in molecular biology. Examples of its manyusesincludecharacterizationofunknownDNA,mutationdetection,alleleidentifi-cation,andcloneconstructconfirmation.Advancesinsequencingtechnology,motivated by the successful International Human Genome Project, have resulted in unprecedented access to high-quality and cost-effective automated analysis. It is increasingly common for researchers to delegate routine DNA sequencing needs to dedicated laboratories or companiesspecializinginthistechnology.Inthisunit,wediscusskeyprotocolsformax-imizing the success of DNA sequencing when working with such service providers and describe detailed troubleshooting advice for analyzing anomalous results.

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Previous	B-14970 Determination of 40 synthetic food colors in drinks and candies by high-performance liquid chromatography using a short column with photodiode array detection	B-14971 Biochemistry, Physiology and Biotechnology of Sulfate-Reducing Bacteria	B-14972 Reagent Preparation: Theoretical and Practical Discussions	B-14973 DNA Sequencing: An Outsourcing Guide	B-14974 Overview of DNA Sequencing Strategies	B-14975 DNA Isolation from Mammalian Samples	B-14976 Wole-Genome Amplification of Single-Cell Genomes for Next-Generation Sequencing	Next

DNA sequencing, the process of determining the precise order of nucleotides in a DNA strand, is a fundamental and ubiquitous method in molecular biology. Examples of its manyusesincludecharacterizationofunknownDNA,mutationdetection,alleleidentifi-cation,andcloneconstructconfirmation.Advancesinsequencingtechnology,motivated by the successful International Human Genome Project, have resulted in unprecedented access to high-quality and cost-effective automated analysis. It is increasingly common for researchers to delegate routine DNA sequencing needs to dedicated laboratories or companiesspecializinginthistechnology.Inthisunit,wediscusskeyprotocolsformax-imizing the success of DNA sequencing when working with such service providers and describe detailed troubleshooting advice for analyzing anomalous results.

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