In vitro induction of triploid roots and shoots from mature endosperm of Jatropha pandureafolia

Tipo de material: Texto

TextoSeries ; Zeitschrift für Pflanzenphysiologie, 66, p.93-96, 1971Trabajos contenidos:

Srivastava, P.S

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Resumen: The mature endosperm of J. panduraefolia proliferated profusely on modified White's medium + 2,4-D + kinetin + YE. The tissue thus obtained grew compact upon continuous subculture and differentiated vascular cells. When callus pieces were transferred to WB + NAA + kinetin + CH, shoot buds and roots differentiated all round the callus. On WB + CH or WB + an auxin, only roots differentiated and on WB + kinetin, only shoots differentiated. In some cultures roots and shoots differentiated from the same callus. Anatomy of the in vitro-differentiated roots and shoots was comparable to in vivo Structures. Cytological preparations of Il-month-old callus showed polyploid cells and chromosomal abnormalities. My results indicate that mature endosperm can also differentiate into triploid organs.

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Previous	B-15500 Structural interactions between viruses as a consequense of mixed infections	B-15501 Cracks in solids	B-15502 Dislocation generation and crack growth under monotonic loading	B-15503 In vitro induction of triploid roots and shoots from mature endosperm of Jatropha pandureafolia	B-15504 Morphogenesis in endosperm cultures	B-15505 Effect of Preincubation of Protoplasts on PEG-induced Fusion of Plant Cells .	B-15506 High frequency induction of somatic embryos in cultured leaf expiants of Coffea arabica L.	Next

The mature endosperm of J. panduraefolia proliferated profusely on modified White's medium + 2,4-D + kinetin + YE. The tissue thus obtained grew compact upon continuous subculture and differentiated vascular cells. When callus pieces were transferred to WB + NAA + kinetin + CH, shoot buds and roots differentiated all round the callus. On WB + CH or WB + an auxin, only roots differentiated and on WB + kinetin, only shoots differentiated. In some cultures roots and shoots differentiated from the same callus. Anatomy of the in vitro-differentiated roots and shoots was comparable to in vivo Structures. Cytological preparations of Il-month-old callus showed polyploid cells and chromosomal abnormalities. My results indicate that mature endosperm can also differentiate into triploid organs.

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