Image from Google Jackets

Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator

Tipo de material: TextoTextoSeries ; Nat Biotechnol., 35(7), p.647-652, 2017Trabajos contenidos:
  • Ajjawi I
  • Verruto J
  • Aqui M
  • Soriaga Lb
  • Coppersmith J
  • Kwok K
  • Peach L
  • Peach L
  • Kalb R
  • Xu W
  • Carlson Tj
  • Francis K
  • Konigsfeld K
  • Bartalis J
  • Schultz A
  • Lambert W
  • Schwartz As
  • Brown R
  • Moellering Er
Recursos en línea: Resumen: Lipid production in the industrial microalga Nannochloropsis gaditana exceeds that of model algal species and can be maximized by nutrient starvation in batch culture. However, starvation halts growth, thereby decreasing productivity. Efforts to engineer N. gaditana strains that can accumulate biomass and overproduce lipids have previously met with little success. We identified 20 transcription factors as putative negative regulators of lipid production by using RNA-seq analysis of N. gaditana during nitrogen deprivation. Application of a CRISPR-Cas9 reverse-genetics pipeline enabled insertional mutagenesis of 18 of these 20 transcription factors. Knocking out a homolog of fungal Zn(II)2Cys6-encoding genes improved partitioning of total carbon to lipids from 20
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Collection Call number Status Date due Barcode
Documentos solicitados Documentos solicitados CICY Documento préstamo interbibliotecario Ref1 B-16657 (Browse shelf(Opens below)) Available

Lipid production in the industrial microalga Nannochloropsis gaditana exceeds that of model algal species and can be maximized by nutrient starvation in batch culture. However, starvation halts growth, thereby decreasing productivity. Efforts to engineer N. gaditana strains that can accumulate biomass and overproduce lipids have previously met with little success. We identified 20 transcription factors as putative negative regulators of lipid production by using RNA-seq analysis of N. gaditana during nitrogen deprivation. Application of a CRISPR-Cas9 reverse-genetics pipeline enabled insertional mutagenesis of 18 of these 20 transcription factors. Knocking out a homolog of fungal Zn(II)2Cys6-encoding genes improved partitioning of total carbon to lipids from 20

There are no comments on this title.

to post a comment.