Conservation of T-DNA in plants regenerated from hairy root cultures

Tipo de material: Texto

TextoSeries ; Bio/Technology, 2(1), p.73-76, 1984Trabajos contenidos:

David, C
Chilton, M.-D
Tempé, J

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Resumen: T1-plants (first generation of transformed plants)regenerated from hairy root culture lines synthesized the same opines and carried T-DNA with the same restriction pattern as the parental line. Opine synthesis segregated in the progeny of T1-plants (T2-plants), Two opine-positive T2-plants had essentially the same T-DNA structure as the parental T1-plant, Clonal constitution of hairy root cultures, facility of regeneration, stability of opine synthesis, and T-DNA structure appear to be valuable attributes of the hairy root system for studies on genetic engineering of plants.

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T1-plants (first generation of transformed plants)regenerated from hairy root culture lines synthesized the same opines and carried T-DNA with the same restriction pattern as the parental line. Opine synthesis segregated in the progeny of T1-plants (T2-plants), Two opine-positive T2-plants had essentially the same T-DNA structure as the parental T1-plant, Clonal constitution of hairy root cultures, facility of regeneration, stability of opine synthesis, and T-DNA structure appear to be valuable attributes of the hairy root system for studies on genetic engineering of plants.

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