Established tools and emerging trends for the production of recombinant proteins and metabolites in Pichia pastoris

Tipo de material: Texto

TextoSeries ; Essays in BioChemistry, 65(2), p.293-307, 2021Trabajos contenidos:

De, S
Mattanovich, D
Ferrer, P
Gasser, B

Tema(s):

Recursos en línea:

Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx

Resumen: Besides bakers' yeast, the methylotrophic yeast Komagataella phaffii (also known as Pichia pastoris)has been developed into the most popular yeast cell factory for the production of heterologous proteins. Strong promoters, stable genetic constructs and a growing collection of freely available strains, tools and protocols have boosted this development equally as thorough genetic and cell biological characterization. This review provides an overview of state-of-the-art tools and techniques for working with P. pastoris, as well as guidelines for the production of recombinant proteins with a focus on small-scale production for biochemical studies and protein characterization. The growing applications of P. pastoris for in vivo biotransformation and metabolic pathway engineering for the production of bulk and specialty chemicals are highlighted as well.

Tags from this library: No tags from this library for this title. Log in to add tags.

Average rating: 0.0 (0 votes)

Holdings
Item type	Current library	Collection	Call number	Status	Date due	Barcode
Documentos solicitados	CICY Documento préstamo interbibliotecario	Ref1	B-19569 (Browse shelf(Opens below))	Available

Browsing CICY shelves, Shelving location: Documento préstamo interbibliotecario, Collection: Ref1 Close shelf browser (Hides shelf browser)

Previous	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	Next
Previous	B-19566 Sensors for process and structural health monitoring of aerospace composites: A review	B-19567 Optimising light conditions increases recombinant protein production in Chlamydomonas reinhardtii chloroplasts	B-19568 Isolation and Transfection of Plant Mesophyll Protoplasts for Virology Research	B-19569 Established tools and emerging trends for the production of recombinant proteins and metabolites in Pichia pastoris	B-1957 Studies of acid-soluble phosphorus compounds in genetically pure lines of Avena fatua with different dormancy characteristics	B-19570 A modified cotton-wool column method for the rapid recovery of DNA from agarose gel slice	B-19571 Comparative analysis of solar - battery storage sizing in netmetering and zero export systems	Next

Besides bakers' yeast, the methylotrophic yeast Komagataella phaffii (also known as Pichia pastoris)has been developed into the most popular yeast cell factory for the production of heterologous proteins. Strong promoters, stable genetic constructs and a growing collection of freely available strains, tools and protocols have boosted this development equally as thorough genetic and cell biological characterization. This review provides an overview of state-of-the-art tools and techniques for working with P. pastoris, as well as guidelines for the production of recombinant proteins with a focus on small-scale production for biochemical studies and protein characterization. The growing applications of P. pastoris for in vivo biotransformation and metabolic pathway engineering for the production of bulk and specialty chemicals are highlighted as well.

There are no comments on this title.

to post a comment.