A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide

Tipo de material: Texto

TextoSeries ; Nature Protocols, 1(5), p.2320-2325, 2006Trabajos contenidos:

Allen, G. C
Flores-Vergara, M. A
Krasynanski, S
Kumar, S
Thompson, W. F

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Resumen: We describe a modification of the DNA extraction method, in which cetyltrimethylammonium bromide (CTAB)is used to extract nucleic acids from plant tissues. In contrast to the original method, the modified CTAB procedure is faster, omits the selective precipitation and CsCl gradient steps, uses less expensive and toxic reagents, requires only inexpensive laboratory equipment and is more readily adapted to high-throughput DNA extraction. This protocol yields approximately 5-30 microg of total DNA from 200 mg of tissue fresh weight, depending on plant species and tissue source. It can be completed in as little as 5-6 h.

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Previous	B-21029 The Agrobacterium tumefaciens Ti plasmid as a host vector system for introducing foreign DNA in plant cells	B-2103 Absorvent product containing a hydrocelloidal composition	B-21030 A Mycological Colour Chart	B-21031 A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide	B-21032 DNA extraction from freeze-dried plant tissue with CTAB in a 96-well format	B-21033 Cetyltrimethyl ammonium bromide (CTAB)DNA miniprep for plant DNA isolation	B-21034 Plant genomic DNA isolation: an art or a science	Next

We describe a modification of the DNA extraction method, in which cetyltrimethylammonium bromide (CTAB)is used to extract nucleic acids from plant tissues. In contrast to the original method, the modified CTAB procedure is faster, omits the selective precipitation and CsCl gradient steps, uses less expensive and toxic reagents, requires only inexpensive laboratory equipment and is more readily adapted to high-throughput DNA extraction. This protocol yields approximately 5-30 microg of total DNA from 200 mg of tissue fresh weight, depending on plant species and tissue source. It can be completed in as little as 5-6 h.

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