DNA extraction from freeze-dried plant tissue with CTAB in a 96-well format

Tipo de material: Texto

TextoSeries ; Cold Spring Harbor Protocols, 2010(11), p.pdb-prot5516, 2010Trabajos contenidos:

Hsia, A. P
Chen, H. D
Ohtsu, K
Schnable, P. S

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Resumen: This protocol is a modified version of DNA isolation using cetyltrimethylammonium bromide (CTAB)and 96-well plates. It is high-throughput, which facilitates the analysis of large mapping populations. The DNA yield is adequate for at least 100-500 polymerase chain reaction (PCR)procedures.

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Previous	B-2103 Absorvent product containing a hydrocelloidal composition	B-21030 A Mycological Colour Chart	B-21031 A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide	B-21032 DNA extraction from freeze-dried plant tissue with CTAB in a 96-well format	B-21033 Cetyltrimethyl ammonium bromide (CTAB)DNA miniprep for plant DNA isolation	B-21034 Plant genomic DNA isolation: an art or a science	B-21035 Carotenoids from Byrsonima crassifolia: Identification, quantification and in vitro scavenging capacity against peroxyl radicals	Next

This protocol is a modified version of DNA isolation using cetyltrimethylammonium bromide (CTAB)and 96-well plates. It is high-throughput, which facilitates the analysis of large mapping populations. The DNA yield is adequate for at least 100-500 polymerase chain reaction (PCR)procedures.

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