Acridonepoxidgehalte in Kalluskulturen von Ruta graveolens und ihre Steigerung durch Mischkultur mit Pilzen

Tipo de material: Texto

TextoSeries ; Z Naturforsch C, 37, p.575-583, 1982Trabajos contenidos:

Wolters, B
Eilert, U

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Resumen: The classic attempt to find optimum culture conditions lead to hormonautotrophic cultures and to dark grown cultures. We made the new attempt to rise the yield of rutacridone-epoxides by coculture with not hostspecific fungi. Conditions were chosen that only diffusible elicitors could influence callus tissue. 14 days of coculture mostly caused reduced acridone-epoxide content, growthrate and up to total disappearance of chlorophyll. Within 3 - 7 days of coculture some fungi induced a significant rise of acridone-epoxide content (up to 50-fold). Culture filtrates of these fungi showed comparable effects. Our results proved the acridone-epoxides to be phytoalexins. The use of fungal elicitors could be a new method to increase secondary product accumulation in tissue culture.

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The classic attempt to find optimum culture conditions lead to hormonautotrophic cultures and to dark grown cultures. We made the new attempt to rise the yield of rutacridone-epoxides by coculture with not hostspecific fungi. Conditions were chosen that only diffusible elicitors could influence callus tissue. 14 days of coculture mostly caused reduced acridone-epoxide content, growthrate and up to total disappearance of chlorophyll. Within 3 - 7 days of coculture some fungi induced a significant rise of acridone-epoxide content (up to 50-fold). Culture filtrates of these fungi showed comparable effects. Our results proved the acridone-epoxides to be phytoalexins. The use of fungal elicitors could be a new method to increase secondary product accumulation in tissue culture.

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