Systematically amino acid analysis: Advancements in extinction coefficient determination for therapeutic proteins

Tipo de material: Texto

TextoSeries Analytical Biochemistry. 690, 115508, 2024, DOI: 10.1016/j.ab.2024.115508Trabajos contenidos:

Xu Y
Shao Z
Zhang L
Cao Y
Liu Z
Zhang Z

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Resumen: Biologicals developers often face challenges in accurately determining the extinction coefficient (EC) measurement. We have successfully improved the precision and robustness of the widely recognized amino acid analysis method for EC determination, through a stepwise optimization process. Extensive analyses based on 114 observations, covering eight proteins over three years were performed, with a maximum relative standard deviation of 1.5% among multiple analysts, and a maximum deviation of 2.8% from the theoretical EC across the eight given proteins examined. © 2024 Elsevier Inc.

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Biologicals developers often face challenges in accurately determining the extinction coefficient (EC) measurement. We have successfully improved the precision and robustness of the widely recognized amino acid analysis method for EC determination, through a stepwise optimization process. Extensive analyses based on 114 observations, covering eight proteins over three years were performed, with a maximum relative standard deviation of 1.5% among multiple analysts, and a maximum deviation of 2.8% from the theoretical EC across the eight given proteins examined. © 2024 Elsevier Inc.

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