The Inoue method for preparation and transformation of competent Escherichia coli:"Ultracompetent" cells

Tipo de material: Texto

TextoSeries Cold Spring Harbor Protocol, 6, p.pdb-prot101196, 2020Trabajos contenidos:

Green, M. R
Sambrook, J

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Resumen: This protocol differs from other transformation procedures in that the bacterial culture is grown at 18 C rather than the conventional 37 C. Otherwise, the protocol is unremarkable and follows a fairly standard course. Under standard laboratory conditions, efficiencies of 1× 10 8 to 3× 10 8 transformed colonies/µg of plasmid DNA are typical.

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Artículo

This protocol differs from other transformation procedures in that the bacterial culture is grown at 18 C rather than the conventional 37 C. Otherwise, the protocol is unremarkable and follows a fairly standard course. Under standard laboratory conditions, efficiencies of 1× 10 8 to 3× 10 8 transformed colonies/µg of plasmid DNA are typical.

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