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Uptake of Plasmid DNA by Protoplasts from Synchronized Soybean Cell Suspension Cultures

Tipo de material: TextoTextoSeries ; Z. Pjlanzenphysiol., 105, p.467-470, 1982Trabajos contenidos:
  • Cress, D.E
Tema(s): Recursos en línea: Resumen: Cell suspension cultur~s of soybean were synchronized by accumulation of cells at the Gil S boundary with 5-fluorodeoxyuridine. Upon removal of fluorodeoxyuridine and addition of thymidine, viable cells resume growth and enter mitosis with increased synchrony. Protoplasts were isolated from cells at varying times following release from inhibition and assayed for uptake of 3H-pBR322 plasmid DNA. Uptake into DNase-resistant, trichloroacetic acid -insoluble form was 2.5 to 4.5-fold higher in S phase than in GI or G2 phase protoplasts. Endo-genous soybean deoxyribonuclease activities did not vary over the cell cycle.
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Documentos solicitados Documentos solicitados CICY Documento préstamo interbibliotecario Ref1 B-15533 (Browse shelf(Opens below)) Available

Cell suspension cultur~s of soybean were synchronized by accumulation of cells at the Gil S boundary with 5-fluorodeoxyuridine. Upon removal of fluorodeoxyuridine and addition of thymidine, viable cells resume growth and enter mitosis with increased synchrony. Protoplasts were isolated from cells at varying times following release from inhibition and assayed for uptake of 3H-pBR322 plasmid DNA. Uptake into DNase-resistant, trichloroacetic acid -insoluble form was 2.5 to 4.5-fold higher in S phase than in GI or G2 phase protoplasts. Endo-genous soybean deoxyribonuclease activities did not vary over the cell cycle.

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