Production of pineapple transgenic plants assisted by temporary immersion bioreactors
Tipo de material:
TextoSeries ; Plant Cell Reports, 21(2), p.136-140, 2002Trabajos contenidos: - Espinosa, P
- Lorenzo, J
- Iglesias, A
- Yabor, L
- Menéndez, E
- Borroto, J
- Hernández, L
- Hernández, L
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Abstract. A procedure for producing pineapple [Ananas comosus (L.)Merr.]transgenic plants was developed that involved selection by micropropagation in temporary immersion bioreactors (TIBs). Pineapple calluses ranging in size from 1.5 mm to 2.0 mm that were co-cultivated with Agrobacterium tumefaciens strains AT2260 (pIG121Hm)and LBA4404 (pTOK233)for 24 h produced the highest percentage (40
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