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A silver staining procedure for nucleic acids in polyacrylamide gels without fixation and pretreatment

Tipo de material: TextoTextoSeries ; Analytical BioChemistry, 391(1), p.77-79, 2009Trabajos contenidos:
  • An, Z.W
  • Xie, L.L
  • Cheng, H
  • Zhou, Y
  • Zhang, Q
  • He, X.G
  • Huang, H.S
Recursos en línea: Resumen: Silver staining of nucleic acid has been widely used in molecular marker analysis such as simple sequence repeat (SSR), single-strand conformation polymorphism (SSCP), and amplified fragment-length polymorphism (AFLP). Many alternatives to silver staining methods have been described, but these methods are not efficient or cost-effective. Here we report a silver staining method that requires less than 10 min for one gel and can save chemicals as well. It has a detection limit of approximately 5.6 pg of DNA/mm2 in nondenaturing polyacrylamide gels and 12.8 pg/mm2 in denaturing polyacrylamide gels.
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Silver staining of nucleic acid has been widely used in molecular marker analysis such as simple sequence repeat (SSR), single-strand conformation polymorphism (SSCP), and amplified fragment-length polymorphism (AFLP). Many alternatives to silver staining methods have been described, but these methods are not efficient or cost-effective. Here we report a silver staining method that requires less than 10 min for one gel and can save chemicals as well. It has a detection limit of approximately 5.6 pg of DNA/mm2 in nondenaturing polyacrylamide gels and 12.8 pg/mm2 in denaturing polyacrylamide gels.

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