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A rapid and efficient transformation method for the production of large numbers of transgenic potato plants

Tipo de material: TextoTextoSeries ; Plant Science, 63(1), p.79-85, 1989Trabajos contenidos:
  • Wenzler, H
  • Mignery, G
  • May, G
  • Park, W
Tema(s): Recursos en línea: Resumen: A procedure is described for the rapid and efficient production of transgenic potato (Solanum tuberosum L.)plants using Agrobacterium-based vectors. The key factors are the genotype and the level of kanamycin sulfate used for selection. Optimized culture conditions easily and routinely permitted the isolation of hundreds of individual regenerates expressing ß-glucuronidase (GUS)transcriptionally fused to promoters from cauliflower mosaic virus (CaMV 35S)or a class-I patatin gene.
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A procedure is described for the rapid and efficient production of transgenic potato (Solanum tuberosum L.)plants using Agrobacterium-based vectors. The key factors are the genotype and the level of kanamycin sulfate used for selection. Optimized culture conditions easily and routinely permitted the isolation of hundreds of individual regenerates expressing ß-glucuronidase (GUS)transcriptionally fused to promoters from cauliflower mosaic virus (CaMV 35S)or a class-I patatin gene.

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