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Selective Isolation of Mercurated DNA by Affinity Chromatography on Thiol Matrices'

Tipo de material: TextoTextoSeries ; Analytical BioChemistry, 146(1), p.64-70, 1985Trabajos contenidos:
  • Banfalvi, G
  • Bhattacharya, S
  • Sarkar, N
Tema(s): Recursos en línea: Resumen: A method for isolating picomole quantities of nascent mercurated DNA from a mixture of cellular nucleic acids using affinity chromatography on thiol-agarose is described. Analysis of mercurated DNA (HgDNA)isolated in the presence of in v-labeled cellular RNA or in vitrosynthesized RNA showed a low level of RNA contamination, about 0.04-0.16
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Documentos solicitados Documentos solicitados CICY Documento préstamo interbibliotecario Ref1 B-12933 (Browse shelf(Opens below)) Available

A method for isolating picomole quantities of nascent mercurated DNA from a mixture of cellular nucleic acids using affinity chromatography on thiol-agarose is described. Analysis of mercurated DNA (HgDNA)isolated in the presence of in v-labeled cellular RNA or in vitrosynthesized RNA showed a low level of RNA contamination, about 0.04-0.16

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