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Development of embryogenic cell suspensions from shoot meristematic tissue in bananas and plantains (Musa spp.)

Tipo de material: TextoTextoSeries ; Plant Science, 170, p.104-112, 2006Trabajos contenidos:
  • Strosse, H
  • Schoofs, H
  • Panis, B
  • Andre, E
  • Reyniers, K
  • Swennen, R
Tema(s): Recursos en línea: Resumen: Multiple meristem cultures of 18 varieties belonging to 5 genome types in Musa (AA, AAA, AAA-h, AAB, ABB)were established by culturing elongated shoots on MS medium supplemented with 100 mM BAP. The top layers comprising the most meristematic tissue, i.e. scalps, were excised and induced for embryogenesis on media containing 1-50 mM 2,4-dichlorophenoxyacetic acid (2,4-D). Embryogenic responses were obtained for each of the tested concentrations, with an optimum at 5 mM 2,4-D. From the 24,375 scalps tested, only 3.3
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Multiple meristem cultures of 18 varieties belonging to 5 genome types in Musa (AA, AAA, AAA-h, AAB, ABB)were established by culturing elongated shoots on MS medium supplemented with 100 mM BAP. The top layers comprising the most meristematic tissue, i.e. scalps, were excised and induced for embryogenesis on media containing 1-50 mM 2,4-dichlorophenoxyacetic acid (2,4-D). Embryogenic responses were obtained for each of the tested concentrations, with an optimum at 5 mM 2,4-D. From the 24,375 scalps tested, only 3.3

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