Electroporation of protoplasts

Tipo de material: Texto

TextoSeries ; Journal of Tissue Culture Methods, 12(4), p.121-126, 1989Trabajos contenidos:

Bates, G.W

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Resumen: A protocol is described for the delivery- of plasmid DNA into plant protoplasts by means of electroporation. Expression of the DNA is monitored 24 h later by a "transient assay" for chloramphenicol acetyltransferase (CATL Stable transformants are recovered by selecting for kanamycin resistance using a modified version of the agarose-bead technique.

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Documentos solicitados	CICY Documento préstamo interbibliotecario	Ref1	B-9940 (Browse shelf(Opens below))	Available

A protocol is described for the delivery- of plasmid DNA into plant protoplasts by means of electroporation. Expression of the DNA is monitored 24 h later by a "transient assay" for chloramphenicol acetyltransferase (CATL Stable transformants are recovered by selecting for kanamycin resistance using a modified version of the agarose-bead technique.

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