PCR-amplification of GC-rich regions: 'slowdown PCR'
PCR-amplification of GC-rich regions: 'slowdown PCR'
- Nature Protocols, 3(8), p.1312-1317, 2008 .
The polymerase chain reaction (PCR)technique has become an indispensable method in molecular research. However, PCR-amplification of GC-rich templates is often hampered by the formation of secondary structures like hairpins and higher melting temperatures. We present a novel method termed 'Slowdown PCR', which allows the successful PCR-amplification of extremely GC-rich (483
The polymerase chain reaction (PCR)technique has become an indispensable method in molecular research. However, PCR-amplification of GC-rich templates is often hampered by the formation of secondary structures like hairpins and higher melting temperatures. We present a novel method termed 'Slowdown PCR', which allows the successful PCR-amplification of extremely GC-rich (483