PCR-amplification of GC-rich regions: 'slowdown PCR' (Record no. 47764)

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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-13565
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Title PCR-amplification of GC-rich regions: 'slowdown PCR'
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Volume/sequential designation Nature Protocols, 3(8), p.1312-1317, 2008
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Summary, etc. The polymerase chain reaction (PCR)technique has become an indispensable method in molecular research. However, PCR-amplification of GC-rich templates is often hampered by the formation of secondary structures like hairpins and higher melting temperatures. We present a novel method termed 'Slowdown PCR', which allows the successful PCR-amplification of extremely GC-rich (483
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Frey, U.H.
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Personal name Bachmann, H.S.
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Personal name Peters, J.
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Personal name Siffert, W.
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Uniform Resource Identifier <a href="https://drive.google.com/file/d/1sRGI3fOEhk7PMmmc5kvZwguaoskiX4Mk/view?usp=drivesdk">https://drive.google.com/file/d/1sRGI3fOEhk7PMmmc5kvZwguaoskiX4Mk/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-13565 25.06.2025 25.06.2025 Documentos solicitados