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PCR-amplification of GC-rich regions: 'slowdown PCR'

Tipo de material: TextoTextoSeries ; Nature Protocols, 3(8), p.1312-1317, 2008Trabajos contenidos:
  • Frey, U.H
  • Bachmann, H.S
  • Peters, J
  • Siffert, W
Recursos en línea: Resumen: The polymerase chain reaction (PCR)technique has become an indispensable method in molecular research. However, PCR-amplification of GC-rich templates is often hampered by the formation of secondary structures like hairpins and higher melting temperatures. We present a novel method termed 'Slowdown PCR', which allows the successful PCR-amplification of extremely GC-rich (483
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Documentos solicitados Documentos solicitados CICY Documento préstamo interbibliotecario Ref1 B-13565 (Browse shelf(Opens below)) Available

The polymerase chain reaction (PCR)technique has become an indispensable method in molecular research. However, PCR-amplification of GC-rich templates is often hampered by the formation of secondary structures like hairpins and higher melting temperatures. We present a novel method termed 'Slowdown PCR', which allows the successful PCR-amplification of extremely GC-rich (483

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