Real-time TaqMan RT-PCR for detection of maize chlorotic mottle virus in maize seeds
Real-time TaqMan RT-PCR for detection of maize chlorotic mottle virus in maize seeds
- Journal of Virological Methods, 171(1), p.292-294, 2011 .
Maize chlorotic mottle virus (MCMV)causes corn lethal necrosis disease, and can be transmitted through infected maize seeds. It remains a challenge to detect this virus in the seeds to prevent its introduction and infection. For this purpose, a real-time TaqMan RT-PCR procedure for efficient detection of MCMV was developed. The sensitivity of the method was 4 fg of total RNA or 25 copies of RNA transcripts, which was approximately ten-fold higher than conventional RT-PCR gel electrophoresis method. The successful detection of MCMV in maize seeds suggested the feasibility of this procedure for routine testing.
MAIZE CHLOROTIC MOTTLE VIRUS
REAL-TIME RT-PCR
QUARANTINE VIRUS
Maize chlorotic mottle virus (MCMV)causes corn lethal necrosis disease, and can be transmitted through infected maize seeds. It remains a challenge to detect this virus in the seeds to prevent its introduction and infection. For this purpose, a real-time TaqMan RT-PCR procedure for efficient detection of MCMV was developed. The sensitivity of the method was 4 fg of total RNA or 25 copies of RNA transcripts, which was approximately ten-fold higher than conventional RT-PCR gel electrophoresis method. The successful detection of MCMV in maize seeds suggested the feasibility of this procedure for routine testing.
MAIZE CHLOROTIC MOTTLE VIRUS
REAL-TIME RT-PCR
QUARANTINE VIRUS