Real-time TaqMan RT-PCR for detection of maize chlorotic mottle virus in maize seeds (Record no. 48601)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01464nam a2200253Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250625153941.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-14411 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250602s9999 xx |||||s2 |||| ||und|d |
| 245 10 - TITLE STATEMENT | |
| Title | Real-time TaqMan RT-PCR for detection of maize chlorotic mottle virus in maize seeds |
| 490 0# - SERIES STATEMENT | |
| Volume/sequential designation | Journal of Virological Methods, 171(1), p.292-294, 2011 |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | Maize chlorotic mottle virus (MCMV)causes corn lethal necrosis disease, and can be transmitted through infected maize seeds. It remains a challenge to detect this virus in the seeds to prevent its introduction and infection. For this purpose, a real-time TaqMan RT-PCR procedure for efficient detection of MCMV was developed. The sensitivity of the method was 4 fg of total RNA or 25 copies of RNA transcripts, which was approximately ten-fold higher than conventional RT-PCR gel electrophoresis method. The successful detection of MCMV in maize seeds suggested the feasibility of this procedure for routine testing. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MAIZE CHLOROTIC MOTTLE VIRUS |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | REAL-TIME RT-PCR |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | QUARANTINE VIRUS |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhang, Yongjiang |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhao, Wenjun |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Li, Mingfu |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chen, Hongjun |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zhu, Shuifang |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1Hks_oBYSQEIv2TZW_DCAGdQLPxd16zdu/view?usp=drivesdk">https://drive.google.com/file/d/1Hks_oBYSQEIv2TZW_DCAGdQLPxd16zdu/view?usp=drivesdk</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 25.06.2025 | B-14411 | 25.06.2025 | 25.06.2025 | Documentos solicitados |