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Real-time TaqMan RT-PCR for detection of maize chlorotic mottle virus in maize seeds

Tipo de material: TextoTextoSeries ; Journal of Virological Methods, 171(1), p.292-294, 2011Trabajos contenidos:
  • Zhang, Yongjiang
  • Zhao, Wenjun
  • Li, Mingfu
  • Chen, Hongjun
  • Zhu, Shuifang
Tema(s): Recursos en línea: Resumen: Maize chlorotic mottle virus (MCMV)causes corn lethal necrosis disease, and can be transmitted through infected maize seeds. It remains a challenge to detect this virus in the seeds to prevent its introduction and infection. For this purpose, a real-time TaqMan RT-PCR procedure for efficient detection of MCMV was developed. The sensitivity of the method was 4 fg of total RNA or 25 copies of RNA transcripts, which was approximately ten-fold higher than conventional RT-PCR gel electrophoresis method. The successful detection of MCMV in maize seeds suggested the feasibility of this procedure for routine testing.
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Maize chlorotic mottle virus (MCMV)causes corn lethal necrosis disease, and can be transmitted through infected maize seeds. It remains a challenge to detect this virus in the seeds to prevent its introduction and infection. For this purpose, a real-time TaqMan RT-PCR procedure for efficient detection of MCMV was developed. The sensitivity of the method was 4 fg of total RNA or 25 copies of RNA transcripts, which was approximately ten-fold higher than conventional RT-PCR gel electrophoresis method. The successful detection of MCMV in maize seeds suggested the feasibility of this procedure for routine testing.

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