Genome editing in plants by engineered CRISPR-Cas9 recognizing NG PAM
Genome editing in plants by engineered CRISPR-Cas9 recognizing NG PAM
- Nature Plants, 5(1), p.14-17, 2019 .
Streptococcus pyogenes Cas9 (SpCas9)is widely used for genome editing and requires NGG as a protospacer adjacent motif (PAM). Here, we show that the engineered SpCas9 (SpCas9-NGv1)can efficiently mutagenize endogenous target sites with NG PAMs in the rice and Arabidopsis genomes. Furthermore, we demonstrate that the SpCas9-NGv1 nickase fused to cytidine deaminase mediates C-to-T substitutions near the 5' end of the target sequence.
CRISPR
Streptococcus pyogenes Cas9 (SpCas9)is widely used for genome editing and requires NGG as a protospacer adjacent motif (PAM). Here, we show that the engineered SpCas9 (SpCas9-NGv1)can efficiently mutagenize endogenous target sites with NG PAMs in the rice and Arabidopsis genomes. Furthermore, we demonstrate that the SpCas9-NGv1 nickase fused to cytidine deaminase mediates C-to-T substitutions near the 5' end of the target sequence.
CRISPR