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Genome editing in plants by engineered CRISPR-Cas9 recognizing NG PAM

Tipo de material: TextoTextoSeries ; Nature Plants, 5(1), p.14-17, 2019Trabajos contenidos:
  • Endo, M
  • Mikami, M
  • Endo, A
  • Kaya, H
  • Itoh, T
  • Nishimasu, H
  • Toki, S
Tema(s): Recursos en línea: Resumen: Streptococcus pyogenes Cas9 (SpCas9)is widely used for genome editing and requires NGG as a protospacer adjacent motif (PAM). Here, we show that the engineered SpCas9 (SpCas9-NGv1)can efficiently mutagenize endogenous target sites with NG PAMs in the rice and Arabidopsis genomes. Furthermore, we demonstrate that the SpCas9-NGv1 nickase fused to cytidine deaminase mediates C-to-T substitutions near the 5' end of the target sequence.
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Streptococcus pyogenes Cas9 (SpCas9)is widely used for genome editing and requires NGG as a protospacer adjacent motif (PAM). Here, we show that the engineered SpCas9 (SpCas9-NGv1)can efficiently mutagenize endogenous target sites with NG PAMs in the rice and Arabidopsis genomes. Furthermore, we demonstrate that the SpCas9-NGv1 nickase fused to cytidine deaminase mediates C-to-T substitutions near the 5' end of the target sequence.

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