Genome editing in plants by engineered CRISPR-Cas9 recognizing NG PAM (Record no. 51536)

MARC details
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fixed length control field 01192nam a2200253Ia 4500
003 - CONTROL NUMBER IDENTIFIER
control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250625160209.0
040 ## - CATALOGING SOURCE
Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-17373
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245 10 - TITLE STATEMENT
Title Genome editing in plants by engineered CRISPR-Cas9 recognizing NG PAM
490 0# - SERIES STATEMENT
Volume/sequential designation Nature Plants, 5(1), p.14-17, 2019
520 3# - SUMMARY, ETC.
Summary, etc. Streptococcus pyogenes Cas9 (SpCas9)is widely used for genome editing and requires NGG as a protospacer adjacent motif (PAM). Here, we show that the engineered SpCas9 (SpCas9-NGv1)can efficiently mutagenize endogenous target sites with NG PAMs in the rice and Arabidopsis genomes. Furthermore, we demonstrate that the SpCas9-NGv1 nickase fused to cytidine deaminase mediates C-to-T substitutions near the 5' end of the target sequence.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CRISPR
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Endo, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Mikami, M.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Endo, A.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Kaya, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Itoh, T.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Nishimasu, H.
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Toki, S.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1HHpkVe27XNeuVaBTw1SL-JVMZOv1C7DY/view?usp=drivesdk">https://drive.google.com/file/d/1HHpkVe27XNeuVaBTw1SL-JVMZOv1C7DY/view?usp=drivesdk</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
Holdings
Lost status Source of classification or shelving scheme Damaged status Not for loan Collection Home library Current library Shelving location Date acquired Total checkouts Full call number Date last seen Price effective from Koha item type
  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 25.06.2025   B-17373 25.06.2025 25.06.2025 Documentos solicitados