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Axenic culture of Lemna trisulca L.

Tipo de material: TextoTextoSeries ; Aquatic Botany, 38(2-3), p.295-301, 1990Trabajos contenidos:
  • Hueberta, David B
  • Mcilraitha, A.L
  • Shaya, J.M
  • Robinson, G.G.C
Recursos en línea: Resumen: Methods for sterilizing, subculturing and achieving sustained growth of Lemna trisulca L. in the laboratory are described. Successful growth and a doubling time of 1.9-2.0 days occurred using inorganic carbon at pH 7.8 as the sole carbon source under 400 µmol m-2s-1 cool white fluorescent light at 28±2°C. Cultures were aerated at a rate of 125 ml min-1 with sterile, humidified, room air. The growth medium was unbuffered and replaced once daily at an exponential rate based on the growth of the plant culture.
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Methods for sterilizing, subculturing and achieving sustained growth of Lemna trisulca L. in the laboratory are described. Successful growth and a doubling time of 1.9-2.0 days occurred using inorganic carbon at pH 7.8 as the sole carbon source under 400 µmol m-2s-1 cool white fluorescent light at 28±2°C. Cultures were aerated at a rate of 125 ml min-1 with sterile, humidified, room air. The growth medium was unbuffered and replaced once daily at an exponential rate based on the growth of the plant culture.

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