PCR-amplification of GC-rich regions: 'slowdown PCR'

Tipo de material: Texto

TextoSeries ; Nature Protocols, 3(8), p.1312-1317, 2008Trabajos contenidos:

Frey, U.H
Bachmann, H.S
Peters, J
Siffert, W

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Resumen: The polymerase chain reaction (PCR)technique has become an indispensable method in molecular research. However, PCR-amplification of GC-rich templates is often hampered by the formation of secondary structures like hairpins and higher melting temperatures. We present a novel method termed 'Slowdown PCR', which allows the successful PCR-amplification of extremely GC-rich (483

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The polymerase chain reaction (PCR)technique has become an indispensable method in molecular research. However, PCR-amplification of GC-rich templates is often hampered by the formation of secondary structures like hairpins and higher melting temperatures. We present a novel method termed 'Slowdown PCR', which allows the successful PCR-amplification of extremely GC-rich (483

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